Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
      In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
    • Involvement in disease
      Pancreatitis, hereditary
      Tropical calcific pancreatitis
    • Sequence similarities
      Contains 1 Kazal-like domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • ISK1_HUMAN antibody
      • Pancreatic secretory trypsin inhibitor antibody
      • Pancreatic serine protease inhibitor Kazal antibody
      • PCTT antibody
      • PSTI antibody
      • Serine protease inhibitor Kazal type 1 antibody
      • Serine protease inhibitor Kazal-type 1 antibody
      • Spink 3 antibody
      • SPINK1 antibody
      • Spink3 antibody
      • TaTI antibody
      • TCP antibody
      • Tumor associated trypsin inhibitor antibody
      • Tumor-associated trypsin inhibitor antibody
      see all

    Anti-SPINK1 antibody images

    • SPINK1 antibody (ab58227) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human pancreas.


    • Observed band size : 29 kDa (why is the actual band size different from the predicted?)
      Western blot against tagged recombinant protein immunogen using ab58227 SPINK1 antibody at 1ug/ml. Predicted band size of immunogen is 29 kDa

    References for Anti-SPINK1 antibody (ab58227)

    This product has been referenced in:
    • Lotan TL  et al. Somatic molecular subtyping of prostate tumors from HOXB13 G84E carriers. Oncotarget 8:22772-22782 (2017). IHC ; Human . Read more (PubMed: 28186998) »
    • Noh BJ  et al. Prognostic value of ERG, PTEN, CRISP3 and SPINK1 in predicting biochemical recurrence in prostate cancer. Oncol Lett 11:3621-3630 (2016). IHC . Read more (PubMed: 27284364) »

    See all 5 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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