The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens. In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labeling SPINK1 with ab183034 at 1/4000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-SPINK1 [EPR12696(2)] antibody (ab183034)
Anti-SPINK1 antibody [EPR12696(2)] (ab183034) at 1/50000 dilution + Human pancreas lysate at 20 µg
Secondary Goat Anti-rabbit IgG HRP at 1/1000 dilution