Recombinant Anti-SPINK1/P12 antibody [EPR12696(2)] (ab183034)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12696(2)] to SPINK1/P12
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SPINK1/P12 antibody [EPR12696(2)]
See all SPINK1/P12 primary antibodies -
Description
Rabbit monoclonal [EPR12696(2)] to SPINK1/P12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human pancreas lysate; Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12696(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
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Notes |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). |
Target
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Function
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production. -
Involvement in disease
Pancreatitis, hereditary
Tropical calcific pancreatitis -
Sequence similarities
Contains 1 Kazal-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6690 Human
- Omim: 167790 Human
- SwissProt: P00995 Human
- Unigene: 407856 Human
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Alternative names
- ISK1_HUMAN antibody
- Pancreatic secretory trypsin inhibitor antibody
- Pancreatic serine protease inhibitor Kazal antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labeling SPINK1/P12 with ab183034 at 1/4000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-SPINK1/P12 antibody [EPR12696(2)] (ab183034) at 1/50000 dilution + Human pancreas lysate at 20 µg
Secondary
Goat Anti-rabbit IgG HRP at 1/1000 dilution
Predicted band size: 9 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab183034 has been referenced in 3 publications.
- Suwa T et al. SPINK1 as a plasma marker for tumor hypoxia and a therapeutic target for radiosensitization. JCI Insight 6:N/A (2021). PubMed: 34747365
- Guo M et al. SPINK1 is a prognosis predicting factor of non-small cell lung cancer and regulates redox homeostasis. Oncol Lett 18:6899-6908 (2019). PubMed: 31788129
- Xu L et al. SPINK1 promotes cell growth and metastasis of lung adenocarcinoma and acts as a novel prognostic biomarker. BMB Rep 51:648-653 (2018). PubMed: 30545439