Recombinant
RabMAb

Anti-SPINK1 antibody [EPR17585-116] (ab207302)

Overview

  • Product name
    Anti-SPINK1 antibody [EPR17585-116]
    See all SPINK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR17585-116] to SPINK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human SPINK1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P00995

  • Positive control
    • WB: Human pancreas lysate. IHC-P: Human pancreas and hepatocellular carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17585-116
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab207302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 8 kDa).

Target

  • Function
    Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
    In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
  • Involvement in disease
    Pancreatitis, hereditary
    Tropical calcific pancreatitis
  • Sequence similarities
    Contains 1 Kazal-like domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ISK1_HUMAN antibody
    • Pancreatic secretory trypsin inhibitor antibody
    • Pancreatic serine protease inhibitor Kazal antibody
    • PCTT antibody
    • PSTI antibody
    • Serine protease inhibitor Kazal type 1 antibody
    • Serine protease inhibitor Kazal-type 1 antibody
    • Spink 3 antibody
    • SPINK1 antibody
    • Spink3 antibody
    • TaTI antibody
    • TCP antibody
    • Tumor associated trypsin inhibitor antibody
    • Tumor-associated trypsin inhibitor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SPINK1 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SPINK1 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-SPINK1 antibody [EPR17585-116] (ab207302) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 8 kDa
    Observed band size: 11 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.

    SPINK1 is a pancreatic secretory trypsin inhibitor, uniquely expressed in pancreas. Human fetal kidney lysate is used as negative control.

    SPINK1 contains a signal peptide, thus observed with a MW of 11 kDa (PMCID: PMC2765331)

References

ab207302 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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