Recombinant
RabMAb

Anti-SPINK1 antibody [EPR17585-30] (ab206294)

Overview

  • Product name
    Anti-SPINK1 antibody [EPR17585-30]
    See all SPINK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR17585-30] to SPINK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • WB; Human pancreas lysate. IHC-P; Human pancreatic carcinoma tissue, Human liver carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17585-30
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab206294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 8 kDa).

Target

  • Function
    Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
    In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
  • Involvement in disease
    Pancreatitis, hereditary
    Tropical calcific pancreatitis
  • Sequence similarities
    Contains 1 Kazal-like domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ISK1_HUMAN antibody
    • Pancreatic secretory trypsin inhibitor antibody
    • Pancreatic serine protease inhibitor Kazal antibody
    • PCTT antibody
    • PSTI antibody
    • Serine protease inhibitor Kazal type 1 antibody
    • Serine protease inhibitor Kazal-type 1 antibody
    • Spink 3 antibody
    • SPINK1 antibody
    • Spink3 antibody
    • TaTI antibody
    • TCP antibody
    • Tumor associated trypsin inhibitor antibody
    • Tumor-associated trypsin inhibitor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling SPINK1 with ab206294 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on human pancreatic carcinoma tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue labeling SPINK1 with ab206294 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on human liver carcinoma tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • All lanes : Anti-SPINK1 antibody [EPR17585-30] (ab206294) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal kidney
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 8 kDa
    Observed band size: 11 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time; Lane 1:5 seconds;
    Lane 2,3 and 4: 3 minutes.

     

     

References

ab206294 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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