Overview

  • Product name
    Anti-SPINK9 antibody
  • Description
    Rabbit polyclonal to SPINK9
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: KQMVDCSHYK KLPPGQQRFC HHMYDPICGS DGKTYKNDCF FCSKVKKTDG TLKFVH, corresponding to amino acids 27-82 of Human SPINK9.

  • Positive control
    • Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Serine protease inhibitor which specifically inhibits KLK5. May contribute to the regulation of the desquamation process in skin by inhibiting KLK5.
  • Tissue specificity
    Skin. Highly expressed at sites of hyperkeratosis. Also detected in thymus, tonsils, testis, pancreas, liver, placenta and brain. Expressed at stratum granulosum and stratum corneum at palmar and plantar sites (at protein level).
  • Sequence similarities
    Contains 1 Kazal-like domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ISK9_HUMAN antibody
    • LEKTI2 antibody
    • Lymphoepithelial Kazal-type-related inhibitor 2 antibody
    • Serine protease inhibitor Kazal-type 9 antibody
    • SPINK9 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122454 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122454, at 1/20 dilution, staining SPINK9 in paraffin embedded Human liver tissue by Immunohistochemistry.

References

ab122454 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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