Anti-EPPIN antibody (ab185699)
Key features and details
- Rabbit polyclonal to EPPIN
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EPPIN antibody -
Description
Rabbit polyclonal to EPPIN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human EPPIN aa 22-133. Mature form.
Database link: O95925 -
Positive control
- THP1, Mouse liver and Mouse testis lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 15 kDa.
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IHC-P |
1/50 - 1/200.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 15 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility. -
Tissue specificity
In testis, expressed and secreted by Sertoli cells, appearing on the surface of testicular and ejaculate spermatozoa. Expressed in the spermatogonia and the earliest preleptotene spermatocytes. In the epididymis, is expressed and secreted by epithelial cells and covers the surface of epididymal spermatozoa and ciliated epithelial cells (at protein level). Expressed specifically in epididymis and testis. Isoform 2 is expressed only in the epididymis. Weak expression is detected in myoid cells as well as spermatogenic cells. -
Sequence similarities
Contains 1 BPTI/Kunitz inhibitor domain.
Contains 1 WAP domain. -
Domain
The BPTI/Kunitz inhibitor domain is required for elastase inhibitory activity. BPTI/Kunitz inhibitor and WAP domains are involved in the protein antibacterial activity. -
Cellular localization
Secreted. Cell surface. Bound to the surface of testicular and on the head and tail of ejaculate spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 100526773 Human
- Entrez Gene: 57119 Human
- Entrez Gene: 75526 Mouse
- Omim: 609031 Human
- SwissProt: O95925 Human
- SwissProt: Q9DA01 Mouse
- Unigene: 121084 Human
- Unigene: 274876 Human
see all -
Alternative names
- Cancer/testis antigen 71 antibody
- CT 71 antibody
- CT71 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling EPPIN with ab185699 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab185699. Blue DAPI for nuclear staining.
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All lanes : Anti-EPPIN antibody (ab185699) at 1/500 dilution
Lane 1 : THP1 cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse testis lysate
Predicted band size: 15 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab185699 has not yet been referenced specifically in any publications.