The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport.
Belongs to the spire family. Contains 1 KIND domain. Contains 3 WH2 domains.
Binds to actin monomers via the WH2 domain. The Spir-box targets binding to intracellular membrane structures.