Overview

  • Product nameAnti-splicing factor 1 antibody
    See all splicing factor 1 primary antibodies
  • Description
    Mouse monoclonal to splicing factor 1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human splicing factor 1

Properties

Applications

Our Abpromise guarantee covers the use of ab58077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.

Target

  • RelevanceSF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BBP antibody
    • D11S636 antibody
    • Mammalian branch point binding protein mBBP antibody
    • OTTHUMP00000069749 antibody
    • OTTHUMP00000069750 antibody
    • OTTHUMP00000069752 antibody
    • Splicing factor 1 antibody
    • Transcription factor ZFM1 antibody
    • ZFM1 antibody
    • Zinc finger gene in MEN1 locus antibody
    • Zinc finger protein 162 antibody
    • ZNF162 antibody
    see all

Anti-splicing factor 1 antibody images

  • Immunofluorescence of  HeLa cells using ab58077 at 10 μg/ml.

  • Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody ab58077 and Protein A Magnetic Bead and immunoblotted with SF1 monoclonal antibody ab58077

  • splicing factor 1 antibody (ab58077) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon.
  • Western blot against tagged recombinant protein immunogen using ab58077 splicing factor 1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

References for Anti-splicing factor 1 antibody (ab58077)

ab58077 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"