Overview

  • Product nameAnti-splicing factor 1 antibody
    See all splicing factor 1 primary antibodies
  • Description
    Rabbit polyclonal to splicing factor 1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2 - 51 (ATGANATPLD FPSKKRKRSR WNQDTMEQKT VIPGMPTVIP PGLTREQERA) of human splicing factor 1 (NP_004621).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • RelevanceSF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BBP antibody
    • D11S636 antibody
    • Mammalian branch point binding protein mBBP antibody
    • OTTHUMP00000069749 antibody
    • OTTHUMP00000069750 antibody
    • OTTHUMP00000069752 antibody
    • Splicing factor 1 antibody
    • Transcription factor ZFM1 antibody
    • ZFM1 antibody
    • Zinc finger gene in MEN1 locus antibody
    • Zinc finger protein 162 antibody
    • ZNF162 antibody
    see all

Anti-splicing factor 1 antibody images

  • Anti-splicing factor 1 antibody (ab83057) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-splicing factor 1 antibody (ab83057)

ab83057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"