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Goat polyclonal to SPO11
ab181495 is expected to recognize both reported isoforms (NP_036576.1; NP_937998.1).
Predicted to work with:
Synthetic peptide corresponding to Human SPO11 aa 128-142 (internal sequence) (Cysteine residue).
Human testis tissue lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 45 kDa).
Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
Highly expressed in testis.
Belongs to the TOP6A family.
Information by UniProt
Cancer/testis antigen 35 antibody
Meiotic recombination protein SPO 11 antibody
Western blot - Anti-SPO11 antibody (ab181495)
Anti-SPO11 antibody (ab181495) at 3 µg/ml + Human testis tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size:
Primary incubation was 1 hour.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"