Overview

  • Product name
  • Description
    Goat polyclonal to SPO11
  • Host species
    Goat
  • Specificity
    ab181495 is expected to recognize both reported isoforms (NP_036576.1; NP_937998.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SPO11 aa 128-142 (internal sequence) (Cysteine residue).
    Sequence:

    NTYATKRDIYYTDSQ


    Database link: NP_036576.1

  • Positive control
    • Human testis tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab181495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
  • Tissue specificity
    Highly expressed in testis.
  • Sequence similarities
    Belongs to the TOP6A family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 35 antibody
    • CT35 antibody
    • Meiotic recombination protein SPO 11 antibody
    • Meiotic recombination protein SPO11 antibody
    • MGC39953 antibody
    • SPATA43 antibody
    • Spermatogenesis associated 43 antibody
    • SPO 11 antibody
    • SPO11 antibody
    • SPO11 meiotic protein covalently bound to DSB antibody
    • SPO11 meiotic protein covalently bound to DSB homolog antibody
    • SPO11 meiotic protein covalently bound to DSB like antibody
    • SPO11_HUMAN antibody
    • TOPVIA antibody
    see all

Images

  • Anti-SPO11 antibody (ab181495) at 3 µg/ml + Human testis tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa



    Primary incubation was 1 hour.

References

ab181495 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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