Overview

  • Product nameAnti-SPO11 antibody
    See all SPO11 primary antibodies
  • Description
    Rabbit polyclonal to SPO11
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human SPO11 aa 108-157 (N terminal).
    Sequence:

    AQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDI


    Database link: NP_036576

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • FunctionRequired for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
  • Tissue specificityHighly expressed in testis.
  • Sequence similaritiesBelongs to the TOP6A family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 35 antibody
    • CT35 antibody
    • Meiotic recombination protein SPO 11 antibody
    • Meiotic recombination protein SPO11 antibody
    • MGC39953 antibody
    • SPATA43 antibody
    • Spermatogenesis associated 43 antibody
    • SPO 11 antibody
    • SPO11 antibody
    • SPO11 meiotic protein covalently bound to DSB antibody
    • SPO11 meiotic protein covalently bound to DSB homolog antibody
    • SPO11 meiotic protein covalently bound to DSB like antibody
    • SPO11_HUMAN antibody
    • TOPVIA antibody
    see all

Anti-SPO11 antibody images

  • Anti-SPO11 antibody (ab81695) (0.2-1 ug/ml) + HepG2 cell lysate

    Predicted band size : 44 kDa
  • Anti-SPO11 antibody (ab81695) at 1 µg/ml + human 293T

    Predicted band size : 44 kDa

References for Anti-SPO11 antibody (ab81695)

This product has been referenced in:
  • Litvinov IV  et al. Ectopic expression of cancer-testis antigens in cutaneous T-cell lymphoma patients. Clin Cancer Res 20:3799-808 (2014). Human . Read more (PubMed: 24850846) »
  • Lindsey SF  et al. Potential role of meiosis proteins in melanoma chromosomal instability. J Skin Cancer 2013:190109 (2013). WB . Read more (PubMed: 23840955) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (Testis spreads-meiotic cells)
Specification Testis spreads-meiotic cells
Permeabilization Yes - Triton X 0.05%
Fixative Paraformaldehyde
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Verified customer

Submitted May 28 2013

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The product ab24355 is the SPO11 peptide that was used as an immunogen for the polyclonal antibody ab22508. This antibody is one of our Fast Track products, and has only been tested in ELISA against the immunizing...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"