The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
RelevanceSPOCK3 may participate in diverse steps of neurogenesis. It inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP and may also interfere with tumor invasion.
Sparc/osteonectin, cwcv and kazal like domains proteoglycan (testican) 3 antibody
SPARC/osteonectin, CWCV, and Kazal like domains proteoglycan 3 antibody
TES 3 antibody
testican 3 antibody
Anti-SPOCK3 antibody images
Western blot - SPOCK3 antibody (ab83713)
Anti-SPOCK3 antibody (ab83713) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta Lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa Observed band size : 49 kDa
References for Anti-SPOCK3 antibody (ab83713)
This product has been referenced in:
Lutter S et al. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation. J Cell Biol197:837-49 (2012).
Read more (PubMed: 22665518) »