Anti-SPON1 antibody - N-terminal (ab170655)

Overview

  • Product name
    Anti-SPON1 antibody - N-terminal
    See all SPON1 primary antibodies
  • Description
    Rabbit polyclonal to SPON1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SPON1 aa 14-44 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9HCB6

  • Positive control
    • Mouse kidney tissue lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 91 kDa.

Target

  • Relevance
    SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.
  • Cellular localization
    Secreted protein; extracellular space; extracellular matrix.
  • Database links
  • Alternative names
    • F spondin antibody
    • SPON 1 antibody
    • spondin 1 (f spondin) extracellular matrix protein antibody
    • Spondin 1 antibody
    • Spondin 1 extracellular matrix protein antibody
    • Spondin 1 precursor antibody
    • Spondin1 antibody
    • Vascular smooth muscle cell growth promoting factor antibody
    • VSGP antibody
    • VSGP/F spondin antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labeling SPON1 with ab170655 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-SPON1 antibody - N-terminal (ab170655) at 1/100 dilution + mouse kidney tissue lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 91 kDa

References

ab170655 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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