The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionInhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the Tdpoz family. Contains 1 BTB (POZ) domain. Contains 1 MATH domain.
DomainThe MATH domain interacts with H2AFY and BMI1.