Recombinant
RabMAb

Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)

Overview

  • Product name
    Anti-SPOP antibody [EPR15771] - C-terminal
    See all SPOP primary antibodies
  • Description
    Rabbit monoclonal [EPR15771] to SPOP - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SPOP aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O43791

  • Positive control
    • PC3, HCT116 and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15771
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.
    • Tissue specificity
      Widely expressed.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the Tdpoz family.
      Contains 1 BTB (POZ) domain.
      Contains 1 MATH domain.
    • Domain
      The MATH domain interacts with H2AFY and BMI1.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BTBD32 antibody
      • HIB homolog 1 antibody
      • Roadkill homolog 1 antibody
      • Speckle type POZ protein antibody
      • Speckle-type POZ protein antibody
      • SPOP antibody
      • SPOP_HUMAN antibody
      • TEF 2 antibody
      • TEF2 antibody
      see all

    Images

    • All lanes : Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilution

      Lane 1 : PC3 cell lysate
      Lane 2 : HCT116 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 42 kDa
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

    • Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    References

    ab192233 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab192233.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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