Synthetic peptide within Human SPOPL aa 228-357 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q6IQ16
Our Abpromise guarantee covers the use of ab171126 in the following tested applications.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 45 kDa.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Flow cytometric analysis of Jurkat cells labeling SPOPL with ab171126 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis in formalin fixed, paraffin embedded Human liver tissue labeling SPOPL with ab171126 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab171126 has not yet been referenced specifically in any publications.