Overview

  • Product name
  • Description
    Rabbit polyclonal to SPOPL
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SPOPL aa 228-357 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6IQ16

  • Positive control
    • UM cell line lysate, Human liver tissue, Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171126 is purified through a protein A column, followed by immuniogen affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    SPOPL belongs to the Tdpoz family. It contains 1 BTB (POZ) domain and 1 MATH domain. In complex with a cullin SPOPL may act in ubiquitination and proteasomal degradation processes.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • HIB homolog 2 antibody
    • Roadkill homolog 2 antibody
    • Speckle type POZ protein like antibody

Images

  • Flow cytometric analysis of Jurkat cells labeling SPOPL with ab171126 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis in formalin fixed, paraffin embedded Human liver tissue labeling SPOPL with ab171126 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-SPOPL antibody (ab171126) at 1/100 dilution + UM cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa

References

ab171126 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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