Overview

  • Product nameAnti-SPOPL antibody
    See all SPOPL primary antibodies
  • Description
    Rabbit polyclonal to SPOPL
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: KGLDDESKDY LSLYLLLVSC PKSEVRAKFK FSLLNAKREE TKAMESQRAY , corresponding to internal sequence amino acids 73-122 of Human SPOPL (NP_001001664).

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab81167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceSPOPL belongs to the Tdpoz family. It contains 1 BTB (POZ) domain and 1 MATH domain. In complex with a cullin SPOPL may act in ubiquitination and proteasomal degradation processes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • HIB homolog 2 antibody
    • Roadkill homolog 2 antibody
    • Speckle type POZ protein like antibody

Anti-SPOPL antibody images

  • Anti-SPOPL antibody (ab81167) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SPOPL antibody (ab81167)

ab81167 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"