Anti-SPRED1 antibody (ab77079)
Key features and details
- Rabbit polyclonal to SPRED1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SPRED1 antibody
See all SPRED1 primary antibodies -
Description
Rabbit polyclonal to SPRED1 -
Host species
Rabbit -
Specificity
ab77079 is predicted to have no cross-reactivity to SPRED2 or SPRED3. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic 14 amino acid peptide from near the center of human SPRED1 (NP_689807).
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Positive control
- Human brain tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. -
Tissue specificity
Weakly expressed in embryonic cell line (HEK-293). -
Involvement in disease
Defects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity. -
Sequence similarities
Contains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain. -
Post-translational
modificationsPhosphorylated on tyrosine. -
Cellular localization
Cell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions. - Information by UniProt
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Database links
- Entrez Gene: 161742 Human
- Entrez Gene: 114715 Mouse
- Entrez Gene: 296072 Rat
- Omim: 609291 Human
- SwissProt: Q7Z699 Human
- SwissProt: Q924S8 Mouse
- Unigene: 525781 Human
- Unigene: 245890 Mouse
see all -
Alternative names
- EVH1 domain-containing protein 1 antibody
- EVH1/Sprouty domain containing protein antibody
- FLJ33903 antibody
see all
Images
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Lane 1 : Anti-SPRED1 antibody (ab77079) at 1 µg/ml
Lane 2 : Anti-SPRED1 antibody (ab77079) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa -
IHC image of ab77079 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77079, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ab77079 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77079 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunofluorescence of Spred1 in Human Brain cells using ab77079 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab77079 has been referenced in 7 publications.
- Liu Y et al. SPOCK2 and SPRED1 function downstream of EZH2 to impede the malignant progression of lung adenocarcinoma in vitro and in vivo. Hum Cell 36:812-821 (2023). PubMed: 36629984
- Susanto A et al. Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation. Exp Eye Res 178:160-175 (2019). PubMed: 30290165
- D'Souza RF et al. Divergent effects of cold water immersion versus active recovery on skeletal muscle fiber type and angiogenesis in young men. Am J Physiol Regul Integr Comp Physiol 314:R824-R833 (2018). PubMed: 29466686
- Wang L et al. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int J Mol Sci 17:N/A (2016). WB ; Mouse . PubMed: 27338342
- Ji JS et al. Inhibition of microRNA-126 promotes the expression of Spred1 to inhibit angiogenesis in hepatocellular carcinoma after transcatheter arterial chemoembolization: in vivo study. Onco Targets Ther 9:4357-67 (2016). IHC-P . PubMed: 27499630
- Cao WJ et al. Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery. Arterioscler Thromb Vasc Biol 35:2401-11 (2015). PubMed: 26381870
- Sharma SB et al. MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol 34:4143-64 (2014). WB ; Human . PubMed: 25202123