• Product nameAnti-SPRED1 antibody [M23-P2G3]
    See all SPRED1 primary antibodies
  • Description
    Mouse monoclonal [M23-P2G3] to SPRED1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from within residues 400 - 500 of Human SPRED1.

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa whole cell, Jurkat whole cell, human brain tissue, mouse brain tissue, rat brain tissue



Our Abpromise guarantee covers the use of ab64740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


  • FunctionTyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
  • Tissue specificityWeakly expressed in embryonic cell line (HEK-293).
  • Involvement in diseaseDefects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
  • Sequence similaritiesContains 1 KBD domain.
    Contains 1 SPR (sprouty) domain.
    Contains 1 WH1 domain.
  • Post-translational
    Phosphorylated on tyrosine.
  • Cellular localizationCell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
  • Information by UniProt
  • Database links
  • Alternative names
    • EVH1 domain-containing protein 1 antibody
    • EVH1/Sprouty domain containing protein antibody
    • FLJ33903 antibody
    • hSpred 1 antibody
    • hSpred1 antibody
    • NFLS antibody
    • PPP1R147 antibody
    • protein phosphatase 1 regulatory subunit 147 antibody
    • SPRE1_HUMAN antibody
    • SPRED 1 antibody
    • Spred-1 antibody
    • spred1 antibody
    • Sprouty related EVH1 domain containing 1 antibody
    • sprouty related EVH1 domain containing protein 1 antibody
    • Sprouty related protein 1 with EVH 1 domain antibody
    • Sprouty-related antibody
    • Suppressor of Ras/MAPK activation antibody
    see all

Anti-SPRED1 antibody [M23-P2G3] images

  • All lanes : Anti-SPRED1 antibody [M23-P2G3] (ab64740) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

    Exposure time : 30 seconds

References for Anti-SPRED1 antibody [M23-P2G3] (ab64740)

This product has been referenced in:
  • Pasmant E  et al. SPRED1, a RAS MAPK pathway inhibitor that causes Legius syndrome, is a tumour suppressor downregulated in paediatric acute myeloblastic leukaemia. Oncogene N/A:N/A (2014). Read more (PubMed: 24469042) »

See 1 Publication for this product

Product Wall

Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in ...

Read More