Anti-Sprouty 1 antibody (ab56670)

Overview

  • Product nameAnti-Sprouty 1 antibody
    See all Sprouty 1 primary antibodies
  • Description
    Mouse monoclonal to Sprouty 1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human Sprouty 1

  • Positive control
    • HepG2 cell lysate, heart tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • Sequence similaritiesBelongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • Cellular localizationCytoplasm. Membrane. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSPRY1 antibody
    • Protein sprouty homolog 1 antibody
    • Sprouty homolog 1 antagonist of FGF signaling antibody
    • Sprouty homolog 1 antibody
    • Spry-1 antibody
    • Spry1 antibody
    • SPY1_HUMAN antibody
    see all

Anti-Sprouty 1 antibody images

  • Overlay histogram showing HepG2 cells stained with ab56670 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56670, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Sprouty 1 antibody (ab56670) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.


  • Predicted band size : 35 kDa
    Sprouty 1 antibody (ab56670) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

References for Anti-Sprouty 1 antibody (ab56670)

ab56670 has not yet been referenced specifically in any publications.

Product Wall

I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

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Thank you very much for your interest our AbTrial program. To our knowledge, our Spouty 1 or Sprouty 2 antibodies have not been tested in flwo cytometry. However by participating in our AbTrial program you can now use our products in an untested app...

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Merci de participer à notre offre de test avec le ab56670 à la place de l'ab73447. Voici le nouveau code pour tester ab56670 chez l'ovin.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"