The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. The detection limit for recombinant tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
Sequence similaritiesBelongs to the sprouty family. Contains 1 SPR (sprouty) domain.
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF.
Ambrosini G et al. Identification of unique MEK-dependent genes in GNAQ mutant uveal melanoma involved in cell growth, tumor cell invasion, and MEK resistance. Clin Cancer Res18:3552-61 (2012).
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