- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Sprouty 2 antibody
See all Sprouty 2 primary antibodies -
DescriptionRabbit polyclonal to Sprouty 2
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Sprouty 2.
(Peptide available as ab101081.)
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Positive control
- This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Lung Tissue; Human Heart Tissue; Jurkat Whole Cell Lysate; Caco2 Whole Cell Lysate; SW480 Whole Cell Lysate. IHC-P: Human skin melanoma FFPE tissue sections
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab85670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionMay function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
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Sequence similaritiesBelongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
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Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF.
- Information by UniProt
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Database links
- Entrez Gene: 10253 Human
- Entrez Gene: 24064 Mouse
- Entrez Gene: 100173576 Orangutan
- Entrez Gene: 306141 Rat
- Omim: 602466 Human
- SwissProt: O43597 Human
- SwissProt: Q9QXV8 Mouse
- SwissProt: Q5R959 Orangutan
see all -
Alternative names
- hSPRY 2 antibody
- hSPRY2 antibody
- MGC23039 antibody
see all
Anti-Sprouty 2 antibody images
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Western blot - Sprouty 2 antibody (ab85670)All lanes : Anti-Sprouty 2 antibody (ab85670) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 35 kDa
Additional bands at : 100 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Sprouty 2 antibody (ab85670)ICC/IF image of ab85670 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85670, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 10µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 2 antibody (ab85670)IHC image of Sprouty 2 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85670, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-Sprouty 2 antibody (ab85670)
ab85670 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"