Our Abpromise guarantee covers the use of ab50317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 19581591|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35 kDa.|
|IP||Use a concentration of 5 - 10 µg/ml.|
|ICC/IF||Use a concentration of 2 - 4 µg/ml.|
ab50317 staining Sprouty 2 in Mouse 8-cell stage pre-implantation embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in 1x PBS and blocked with 3% BSA for 4 hours 30 minutes at 4°C. Samples were incubated with primary antibody (1/300 in 1 x PBS + 3% BSA + 0.15% Tween-20) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
The image of an 8-cell stage pre-implantation Mouse embryo that was injected with wtSpry2 (N-terminally tagged with YFP) mRNA at 2-cell stage (in green) and stained with anti-Spry2 antibody (ab50317) (in red). Nuclei are stained with DAPI (in blue).