Immunocytochemistry/ Immunofluorescence - Anti-Sprouty 2 antibody - Aminoterminal end (ab50317)This image is courtesy of an Abreview submitted by Aleksandar Mihajlovic
ab50317 staining Sprouty 2 in Mouse 8-cell stage pre-implantation embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in 1x PBS and blocked with 3% BSA for 4 hours 30 minutes at 4°C. Samples were incubated with primary antibody (1/300 in 1 x PBS + 3% BSA + 0.15% Tween-20) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
The image of an 8-cell stage pre-implantation Mouse embryo that was injected with wtSpry2 (N-terminally tagged with YFP) mRNA at 2-cell stage (in green) and stained with anti-Spry2 antibody (ab50317) (in red). Nuclei are stained with DAPI (in blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sprouty 2 antibody - Aminoterminal end (ab50317)This image is courtesy of an Abreview submitted by Imran Ahmad
ab50317 staining Sprouty 2 in Mouse bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 hours at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/500) for 24 hours at 4°C. An undiluted HRP-conjugated Mouse anti-rabbit polyclonal was used as the secondary antibody.