Recombinant
RabMAb

Anti-Sprouty 2 antibody [EPR4318(2)(B)] (ab180527)

Overview

  • Product name
    Anti-Sprouty 2 antibody [EPR4318(2)(B)]
    See all Sprouty 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4318(2)(B)] to Sprouty 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sprouty 2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O43597

  • Positive control
    • HT-29, PC-3 and HeLa cell lysates; SH-SY5Y cells and cell lysates; Human breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50.
ICC/IF 1/100.
IP 1/40.

Target

  • Function
    May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • Sequence similarities
    Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • Domain
    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF.
  • Information by UniProt
  • Database links
  • Alternative names
    • hSPRY 2 antibody
    • hSPRY2 antibody
    • MGC23039 antibody
    • Protein sprouty homolog 2 antibody
    • Sprouty 2 antibody
    • Sprouty homolog 2 (Drosophila) antibody
    • Sprouty homolog 2 antibody
    • Sprouty2 antibody
    • Spry 2 antibody
    • Spry-2 antibody
    • SPRY2 antibody
    • SPY2_HUMAN antibody
    see all

Images

  • Western blot analysis of Hela cell lysate immunoprecipitated with ab180527. Detection utilised ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. The blocking buffer and dilution buffer was 5% NFDM/TBST.

  • Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Sprouty 2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.

     

     

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Sprouty 2 with ab180527 at 1/50 dilution. The slide is counterstained with Hematoxylin.

  • All lanes : Anti-Sprouty 2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • All lanes : Anti-Sprouty 2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

References

ab180527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180527.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up