Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionSuppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
Sequence similaritiesBelongs to the sprouty family. Contains 1 SPR (sprouty) domain.
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
ab115557, at 2.5µg/ml, staining Sprouty 4 in Formalin-fixed, Paraffin-embedded Human heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-Sprouty 4 antibody (ab115557)
has not yet been referenced specifically in any publications.
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