Overview

  • Product name
  • Description
    Rabbit polyclonal to Sprouty 4
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CKAASGDAKTSRPDKPF

    conjugated to KLH, corresponding to C terminal amino acids 306-322 of Human Sprouty 4 (NP_112226.2).

  • Positive control
    • Human Heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/3000 - 1/12000.
WB 1/100 - 1/1000. Predicted molecular weight: 33 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
  • Sequence similarities
    Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • Domain
    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • Cellular localization
    Cytoplasm. Cell projection > ruffle membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein sprouty homolog 4 antibody
    • Sprouty homolog 4 antibody
    • sprouty homolog 4 (Drosophila) antibody
    • SPRY 4 antibody
    • Spry-4 antibody
    • Spry4 antibody
    • SPY4_HUMAN antibody
    see all

Images

  • ab115557, at 2.5µg/ml, staining Sprouty 4 in Formalin-fixed, Paraffin-embedded Human heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
Yes - Casblock
Specification
Pancreas
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Dror Kolodkin-Gal

Verified customer

Submitted Feb 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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