Overview

  • Product nameAnti-Sprouty 4 antibody
    See all Sprouty 4 primary antibodies
  • Description
    Rabbit polyclonal to Sprouty 4
  • SpecificityThis antibody reacts specifically with human 32 kDa Sprouty 4 protein.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide derived from N terminal region of human Sprouty 4.

Properties

Applications

Our Abpromise guarantee covers the use of ab59785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 32 kDa.

Target

References for Anti-Sprouty 4 antibody (ab59785)

This product has been referenced in:
  • Barbáchano A  et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . Read more (PubMed: 20543868) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (8-cell stage mouse pre-implantation embryo)
Specification 8-cell stage mouse pre-implantation embryo
Permeabilization Yes - 0.5% Triton X-100 in 1xPBS
Fixative Paraformaldehyde
Username

Aleksandar Mihajlovic

Verified customer

Submitted Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (skin wound)
Loading amount 15 µg
Specification skin wound
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Precedia Massey

Verified customer

Submitted Feb 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"