This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody has been tested by Western blot against cell lysates. We believe that Sprouty 4 is represented by the doublet at ~ 35kDa. We believe that this protein may require IP before Western blotting, corresponding to results seen in Sasaki et al. We have not yet succeeded in peptide blocking the bands detected with this antibody.

  • Target

    • Function
      Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
    • Sequence similarities
      Belongs to the sprouty family.
      Contains 1 SPR (sprouty) domain.
    • Domain
      The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
    • Cellular localization
      Cytoplasm. Cell projection > ruffle membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein sprouty homolog 4 antibody
      • Sprouty homolog 4 antibody
      • sprouty homolog 4 (Drosophila) antibody
      • SPRY 4 antibody
      • Spry-4 antibody
      • Spry4 antibody
      • SPY4_HUMAN antibody
      see all

    Anti-Sprouty 4 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Western blot using ab7513 at 1/500.
       
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
      Exposure time: 2minutes.
      Lysates at 5µg per lane.

      We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

    • All lanes : Anti-Sprouty 4 antibody (ab7513) at 1/100 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SW13 whole cell lysate
      Lane 3 : C2C12 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.
    • ab7513 at 2.5 µg/ml staining human heart tissue.

    • ab7513 staining tumour cells in human liver tissue.  Brown color indicates presence of protein, blue color shows cell nuclei.

    References for Anti-Sprouty 4 antibody (ab7513)

    This product has been referenced in:
    • So WK  et al. Sprouty4 mediates amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells. Tumour Biol 37:9197-207 (2016). Read more (PubMed: 26768617) »
    • Barbáchano A  et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . Read more (PubMed: 20543868) »

    See all 3 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma)
    Loading amount
    500 µg
    Specification
    Melanoma
    Gel Running Conditions
    Reduced Denaturing (15 %)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 06 2010

    Thank you for your enquiry. Ab7513 is a Fast-Track antibody and has not yet been fully characterized. Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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