Recombinant
RabMAb

Anti-Sprouty 4 antibody [EPR12127] (ab176337)

Overview

  • Product name
    Anti-Sprouty 4 antibody [EPR12127]
    See all Sprouty 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR12127] to Sprouty 4
  • Tested applications
    Suitable for: Flow Cyt, IP, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sprouty 4 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9C004

  • Positive control
    • HepG2, MCF7, HeLa, and K562 cell lysates. HeLa cells and permeabilized K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12127
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
    • Sequence similarities
      Belongs to the sprouty family.
      Contains 1 SPR (sprouty) domain.
    • Domain
      The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
    • Cellular localization
      Cytoplasm. Cell projection > ruffle membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein sprouty homolog 4 antibody
      • Sprouty homolog 4 antibody
      • sprouty homolog 4 (Drosophila) antibody
      • SPRY 4 antibody
      • Spry-4 antibody
      • Spry4 antibody
      • SPY4_HUMAN antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of permeabilized K562 cells labeling Sprouty 4 with ab176337 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunofluorescent analysis of HeLa cells labeling Sprouty 4 with ab176337 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • All lanes : Anti-Sprouty 4 antibody [EPR12127] (ab176337) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 33 kDa

    References

    ab176337 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176337.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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