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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sprouty 4 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9C004
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab176337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/50 - 1/100.|
Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized K562 cells labeling Sprouty 4 with ab176337 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of HeLa cells labeling Sprouty 4 with ab176337 at 1/50 dilution (green). DAPI nuclear staining (blue).
ab176337 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"