1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionSuppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
Sequence similaritiesBelongs to the sprouty family. Contains 1 SPR (sprouty) domain.
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
ab103114, at 1/50 dilution, staining Sprouty 4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
Western blot - Anti-Sprouty 4 (phospho Y75) antibody (ab103114)
Anti-Sprouty 4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg
Predicted band size : 32 kDa
References for Anti-Sprouty 4 (phospho Y75) antibody (ab103114)
has not yet been referenced specifically in any publications.
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