Overview

  • Product name
    Anti-Sprouty 4 (phospho Y75) antibody
    See all Sprouty 4 primary antibodies
  • Description
    Rabbit polyclonal to Sprouty 4 (phospho Y75)
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 57-86 from Tyrosine 75 region of Human Sprouty 4

  • Positive control
    • K-562 cell lysate. Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
  • Sequence similarities
    Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • Domain
    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • Cellular localization
    Cytoplasm. Cell projection > ruffle membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein sprouty homolog 4 antibody
    • Sprouty homolog 4 antibody
    • sprouty homolog 4 (Drosophila) antibody
    • SPRY 4 antibody
    • Spry-4 antibody
    • Spry4 antibody
    • SPY4_HUMAN antibody
    see all

Anti-Sprouty 4 (phospho Y75) antibody images

  • ab103114, at 1/50 dilution, staining Sprouty 4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • Anti-Sprouty 4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 32 kDa

References for Anti-Sprouty 4 (phospho Y75) antibody (ab103114)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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