The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
Tissue specificitySuprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
Sequence similaritiesBelongs to the cornifin (SPRR) family.
Developmental stageExpressed during differentiation of squamous cells.
Post-translational modificationsThe N-terminus is blocked.
Immunohistochemistry (Frozen sections) analysis of mouse eyeball sections labelling SPRR1b with ab123237 at 1:50. An Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:50) was used as the secondary antibody.
Western blot - Anti-SPRR1b antibody (ab123237)
Anti-SPRR1b antibody (ab123237) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary Goat anti-Rabbit IgG HRP
Predicted band size : 10 kDa Gel concentration: 10-20%.
References for Anti-SPRR1b antibody (ab123237)
This product has been referenced in:
Fang Z et al. Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins. BMC Cancer16:706 (2016).
Read more (PubMed: 27586393) »