Overview

  • Product nameAnti-SPRR2F antibody
  • Description
    Mouse monoclonal to SPRR2F
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-72 of Human SPRR2F with proprietary tag (NP_001014450).

  • Positive control
    • SPRR2F transfected 293T cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab118130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 8 kDa.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionCross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
  • Sequence similaritiesBelongs to the cornifin (SPRR) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000016115 antibody
    • Small proline-rich protein 2F antibody
    • SPR-2F antibody
    • SPR2F_HUMAN antibody
    • SPRR2F antibody
    see all

Anti-SPRR2F antibody images

  • Anti-SPRR2F antibody (ab118130) at 5 µg/ml + recombinant immunogen at 0.2 µg

    Predicted band size : 8 kDa
  • All lanes : Anti-SPRR2F antibody (ab118130) at 5 µg/ml

    Lane 1 : 293T cell line lysate, transfected with SPRR2F
    Lane 2 : 293T cell line lysate, non-transfected

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 8 kDa
  • Detection limit for recombinant tagged SPRR2F is 0.1 ng/ml as a capture antibody.

References for Anti-SPRR2F antibody (ab118130)

ab118130 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118130.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"