Overview

  • Product name
  • Description
    Rabbit polyclonal to SPRYD5
  • Specificity
    ab113101 is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist; ab113101 will detect both isoforms.
  • Tested applications
    Suitable for: WB, ICC/IF, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide containing an 18 amino acid sequence from the C terminal of Human SPRYD5 (NP_116070).

  • Positive control
    • Rat brain tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab113101 is purified by affinity chromatography via a peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab113101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 52 kDa.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC10977 antibody
    • SPRY domain containing 5 antibody
    • SPRY domain containing protein 5 antibody
    • SPRY domain-containing protein 5 antibody
    • SPRY5_HUMAN antibody
    • SPRYD5 antibody
    • TRIM51 antibody
    • TRIM51A antibody
    • Tripartite motif containing 51 antibody
    • Tripartite motif containing protein 51 antibody
    • Tripartite motif-containing protein 51 antibody
    see all

Images

  • Immunofluorescence of SPRYD5 in mouse brain tissue with ab113101 at 20 ug/mL.
  • Lane 1 : Anti-SPRYD5 antibody (ab113101) at 0.25 µg/ml
    Lane 2 : Anti-SPRYD5 antibody (ab113101) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 52 kDa
  • Immunohistochemical analysis of mouse brain tissue, staining SPRYD5 with ab113101 at 5 µg/ml.

References

ab113101 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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