Overview

  • Product nameAnti-SPSB2 antibody
    See all SPSB2 primary antibodies
  • Description
    Rabbit polyclonal to SPSB2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide (IGRGKLYHQS KGPGAPQYPA GTQGEQLEVP ERLLVVLDME EGTLGYAIGG) corresponding to a region within C terminal amino acids 135-184 of Human SPSB2 (NP_116030).

  • Positive control
    • COLO205 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionProbable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the SPSB family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 SOCS box domain.
  • DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ17395 antibody
    • Gene-rich cluster protein C9 antibody
    • GRCC9 antibody
    • MGC2519 antibody
    • OTTHUMP00000225007 antibody
    • OTTHUMP00000225008 antibody
    • splA/ryanodine receptor domain and SOCS box containing 2 antibody
    • SPRY domain containing SOCS box protein 2 antibody
    • SPRY domain containing SOCS box protein SSB 2 antibody
    • SPRY domain-containing SOCS box protein 2 antibody
    • Spsb2 antibody
    • SPSB2_HUMAN antibody
    • SSB-2 antibody
    • SSB2 antibody
    see all

Anti-SPSB2 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of Rhesus macaque spinal cord labeling SPSB2 using ab113778 at 1:300. An Alexa Fluor® 488-conjugated Donkey anti-rabbit (1:500) was used as the secondary antibody.

  • Anti-SPSB2 antibody (ab113778) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Predicted band size : 29 kDa
    Gel concentration: 12%

References for Anti-SPSB2 antibody (ab113778)

ab113778 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"