Overview

  • Product name
    Anti-SPT3 antibody
  • Description
    Rabbit polyclonal to SPT3
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment corresponding to the N terminal domain (1-200aa) of Saccharomyces cerevisiae SPT3, purified by chromatography.

  • Positive control
    • Saccharomyces cerevisiae extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab63811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    SPT factors 3, 7 and 8 are required for the initiation of Ty transcription from the delta promoter. SPT3 regulates Ty1 as well as the mating factor genes. It interacts directly with TATA-binding protein, and is likely to be required for normal TBP function at a subset of RNA polymerase II-dependent promoters.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Positive regulator of Ty transcription antibody
      • SPT3 antibody
      • SPT3 like protein antibody
      • Spt3p antibody
      • SUPT3H antibody
      • Transcription initiation protein SPT3 homolog antibody
      • YDR392W antibody
      see all

    Images

    • All lanes : Anti-SPT3 antibody (ab63811) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Saccharomyces cerevisiae extract


      Predicted band size : 39 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Lin L  et al. Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4. Proc Natl Acad Sci U S A 109:1997-2002 (2012). WB ; Saccharomyces cerevisiae . Read more (PubMed: 22308403) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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