The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
Use at an assay dependent dilution.
RelevanceSPT factors 3, 7 and 8 are required for the initiation of Ty transcription from the delta promoter. SPT3 regulates Ty1 as well as the mating factor genes. It interacts directly with TATA-binding protein, and is likely to be required for normal TBP function at a subset of RNA polymerase II-dependent promoters.
Lin L et al. Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4. Proc Natl Acad Sci U S A109:1997-2002 (2012).
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