Overview

  • Product nameAnti-SPT3 antibody
    See all SPT3 primary antibodies
  • Description
    Rabbit polyclonal to SPT3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: GARVITPEDL LFLMRKDKKK LRRLLKYMFI RDYKSKIVKG IDEDDLLEDK, corresponding to amino acids 74-123 of Human SPT3

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 23, 60 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-SPT3 antibody images

  • Anti-SPT3 antibody (ab66892) at 0.4 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of Human Liver cell lysate tissue at an antibody concentration of 4-8µg/ml using anti-SUPT3H antibody (ab66892)

References for Anti-SPT3 antibody (ab66892)

ab66892 has not yet been referenced specifically in any publications.

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