Anti-SPTBN1 antibody (ab72239)
Key features and details
- Rabbit polyclonal to SPTBN1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPTBN1 antibody
See all SPTBN1 primary antibodies -
Description
Rabbit polyclonal to SPTBN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human SPTBN1.
Database link: Q01082 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: HeLa cells. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
Tissue specificity
Isoform 2 is present in brain, lung and kidney (at protein level). -
Sequence similarities
Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats. -
Post-translational
modificationsIsoform 2 is phosphorylated on Ser-8 and Ser-10. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes. - Information by UniProt
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Database links
- Entrez Gene: 526027 Cow
- Entrez Gene: 6711 Human
- Entrez Gene: 716794 Rhesus monkey
- Omim: 182790 Human
- SwissProt: Q01082 Human
- Unigene: 503178 Human
- Unigene: 705692 Human
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Alternative names
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SPTBN1 with ab72239 at 1/1000 (0.2µg/ml). Detection: DAB.
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All lanes : Anti-SPTBN1 antibody (ab72239) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 274 kDa
Observed band size: 274 kDa -
Immunoprecipitation/ Western Blot of SPTBN1.
Lane 1: ab72239 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72239 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 1 second. -
IHC image of ab72239 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72239, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab72239 has been referenced in 10 publications.
- Choi C et al. Analyzing the mechanisms that facilitate the subtype-specific assembly of γ-aminobutyric acid type A receptors. Front Mol Neurosci 15:1017404 (2022). PubMed: 36263376
- Xu X et al. SPTBN1 Prevents Primary Osteoporosis by Modulating Osteoblasts Proliferation and Differentiation and Blood Vessels Formation in Bone. Front Cell Dev Biol 9:653724 (2021). PubMed: 33816505
- Ma X et al. β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia. J Cell Mol Med 25:11300-11309 (2021). PubMed: 34799994
- Yang P et al. miR-153-3p Targets βII Spectrin to Regulate Formaldehyde-Induced Cardiomyocyte Apoptosis. Front Cardiovasc Med 8:764831 (2021). PubMed: 34977182
- Smalley JL et al. Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes. Front Mol Neurosci 13:563091 (2020). PubMed: 33192291
- Liang L et al. Validation of a multi-omics strategy for prioritizing personalized candidate driver genes Oncotarget 7:38440-38450 (2016). PubMed: 27469031
- Ji F et al. The prognostic value of combined TGF-ß1 and ELF in hepatocellular carcinoma. BMC Cancer 15:116 (2015). IHC . PubMed: 25880619
- Kiriyama S et al. CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma. Ann Surg Oncol 21 Suppl 4:S505-14 (2014). PubMed: 24390710
- Forster F et al. Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton. J Immunol 192:771-81 (2014). PubMed: 24337748
- Dai L et al. IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation. J Exp Med 210:517-34 (2013). PubMed: 23460728