Overview

  • Product name
  • Description
    Rabbit polyclonal to SPTBN1
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 2100 and 2150 of human SPTBN1 (NP_003119.2)

  • Positive control
    • Whole cell lysate from Hela and 293T cells. IF: A431 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab72239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Tissue specificity
    Isoform 2 is present in brain, lung and kidney (at protein level).
  • Sequence similarities
    Belongs to the spectrin family.
    Contains 2 CH (calponin-homology) domains.
    Contains 1 PH domain.
    Contains 17 spectrin repeats.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated on Ser-8 and Ser-10.
  • Cellular localization
    Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta G spectrin antibody
    • Beta II spectrin antibody
    • Beta spectrin 2 antibody
    • Beta spectrin II antibody
    • Beta-II spectrin antibody
    • betaSpII antibody
    • brain 1 antibody
    • ELF antibody
    • Embryonic liver beta fodrin antibody
    • Fodrin beta chain antibody
    • non-erythroid beta chain 1 antibody
    • Spectrin antibody
    • Spectrin beta chain antibody
    • Spectrin, beta, non erythrocytic 1 antibody
    • Spectrin, non erythroid beta chain 1 antibody
    • SPTB2 antibody
    • SPTB2_HUMAN antibody
    • SPTBN1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SPTBN1 with ab72239 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-SPTBN1 antibody (ab72239) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 274 kDa
    Observed band size : 274 kDa
  • Immunoprecipitation/ Western Blot of SPTBN1.
    Lane 1: ab72239 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72239 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 1 second.
  • IHC image of ab72239 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72239, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab72239 stained A431 cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72239, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ji F  et al. The prognostic value of combined TGF-ß1 and ELF in hepatocellular carcinoma. BMC Cancer 15:116 (2015). IHC . Read more (PubMed: 25880619) »
  • Kiriyama S  et al. CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma. Ann Surg Oncol 21 Suppl 4:S505-14 (2014). Read more (PubMed: 24390710) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (Neonatal Rat Ventricular Myocytes)
Loading amount
40 µg
Specification
Neonatal Rat Ventricular Myocytes
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. David Grubb

Verified customer

Submitted Mar 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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