The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Tissue specificityIsoform 2 is present in brain, lung and kidney (at protein level).
Sequence similaritiesBelongs to the spectrin family. Contains 2 CH (calponin-homology) domains. Contains 1 PH domain. Contains 17 spectrin repeats.
Post-translational modificationsIsoform 2 is phosphorylated on Ser-8 and Ser-10.
Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.