Overview

  • Product name
  • Description
    Rabbit polyclonal to SPZ1
  • Specificity
    This antibody reacts with SPZ1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    A region within synthetic peptide: FKHTTRATEG TIRKPKILRS PDVCFTKKAR NNRFNARVAK KSLVGKRRTV, corresponding to C terminal amino acids 325-374 of Mouse SPZ1

  • Positive control
    • NIH3T3 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay: 1/312500.

Target

  • Relevance
    SPZ1 is a novel bHLH-Zip protein.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • BHLH Zip transcription factor SPZ1 antibody
    • FLJ25709 antibody
    • MGC133987 antibody
    • NYD TSP1 antibody
    • PP1C gamma 2 antibody
    • PP1C gamma antibody
    • PPP1R148 antibody
    • Protein phosphatase 1, regulatory subunit 148 antibody
    • Spermatogenic leucine zipper 1 antibody
    • Spermatogenic leucine zipper protein 1 antibody
    • Spermatogenic Zip 1 antibody
    • Spz1 antibody
    • Testis secretory sperm-binding protein Li 232m antibody
    • Testis specific protein 1 antibody
    • Testis specific protein NYD TSP1 antibody
    • TSP1 antibody
    see all

Images

  • Anti-SPZ1 antibody (ab50870) at 2.5 µg/ml + NIH3T3 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References

ab50870 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50870.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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