The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 24501170
FunctionCalcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
Tissue specificityDetected in cardiac myocytes.
Sequence similaritiesContains 4 EF-hand domains.
Cellular localizationCytoplasm. Sarcoplasmic reticulum membrane. Relocates to the sarcoplasmic reticulum membrane in response to elevated calcium levels.