Recombinant
RabMAb

Anti-srGAP2 antibody [EP2506(2)] (ab124958)

Overview

  • Product name
    Anti-srGAP2 antibody [EP2506(2)]
    See all srGAP2 primary antibodies
  • Description
    Rabbit monoclonal [EP2506(2)] to srGAP2
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human srGAP2.

  • Positive control
    • Mouse brain, SH SY5Y, 293T and JAR lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : SH SY5Y lysates
      Lane 3 : 293T lysates
      Lane 4 : JAR lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 121 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Sample
    Mouse Tissue sections (mouse embryonic spinal cord E11)
    Specification
    mouse embryonic spinal cord E11
    Permeabilization
    Yes - 0,25%TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Alain Chedotal

    Verified customer

    Submitted May 17 2013

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS gradient gel)
    Sample
    Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
    Specification
    HEK293H, COS7, mouse hindbrain lysate E14
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Alain Chedotal

    Verified customer

    Submitted May 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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