Overview

  • Product name
    Anti-SRA1 antibody
    See all SRA1 primary antibodies
  • Description
    Rabbit polyclonal to SRA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from a region between residue 180 and the C-terminus (residue 237) of human SRA1, NP_001030312.1.

  • Positive control
    • Whole cell lysate from Hela, MCF-7 and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • Tissue specificity
    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • Sequence similarities
    Belongs to the SRA1 family.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • MGC87674 antibody
      • PP7684 antibody
      • SR A1 antibody
      • SRA antibody
      • SRA1 antibody
      • SRA1_HUMAN antibody
      • SRAP antibody
      • Steroid receptor coactivator antibody
      • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
      • Steroid receptor RNA activator 1 antibody
      • Steroid receptor RNA activator protein antibody
      • STRAA1 antibody
      see all

    Images

    • Western blot - SRA1 antibody (ab72407)
      Western blot - SRA1 antibody (ab72407)
      All lanes : Anti-SRA1 antibody (ab72407) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 20 µg
      Lane 2 : Whole cell lysate from Hela cells at 4 µg
      Lane 3 : Whole cell lysate from MCF-7 cells at 20 µg
      Lane 4 : Whole cell lysate from 293T cells at 20 µg

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa (why is the actual band size different from the predicted?)


      Exposure time: 5 minutes
    • Immunoprecipitation - SRA1 antibody (ab72407)
      Immunoprecipitation - SRA1 antibody (ab72407)
      Immunoprecipitation/ Western Blot of SRA1.
      Lane 1: ab72407 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72407 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 10 seconds.

    References

    This product has been referenced in:
    • McKay DB  et al. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. J Mol Biol 426:1766-85 (2014). IF . Read more (PubMed: 24486609) »

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