Anti-SRA1 antibody (ab72407)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-SRA1 antibody
See all SRA1 primary antibodies - DescriptionRabbit polyclonal to SRA1
- Tested applicationsSuitable for: WB, IPmore details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide from a region between residue 180 and the C-terminus (residue 237) of human SRA1, NP_001030312.1.
- Positive control
- Whole cell lysate from Hela, MCF-7 and 293T cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
- FunctionFunctional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
- Tissue specificityHighly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
- Sequence similaritiesBelongs to the SRA1 family.
- Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 10011 Human
- Omim: 603819 Human
- SwissProt: Q9HD15 Human
- Unigene: 653135 Human
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Alternative names
- MGC87674 antibody
- PP7684 antibody
- SR A1 antibody
see all
Anti-SRA1 antibody images
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Western blot - SRA1 antibody (ab72407)All lanes : Anti-SRA1 antibody (ab72407) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 20 µg
Lane 2 : Whole cell lysate from Hela cells at 4 µg
Lane 3 : Whole cell lysate from MCF-7 cells at 20 µg
Lane 4 : Whole cell lysate from 293T cells at 20 µg
Developed using the ECL technique
Predicted band size : 26 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes -
Immunoprecipitation - SRA1 antibody (ab72407)Immunoprecipitation/ Western Blot of SRA1.
Lane 1: ab72407 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72407 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 10 seconds.
References for Anti-SRA1 antibody (ab72407)
This product has been referenced in:
- McKay DB et al. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. J Mol Biol 426:1766-85 (2014). IF . Read more (PubMed: 24486609) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"