The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Use at 2-5 µg/mg of lysate.
FunctionFunctional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
Tissue specificityHighly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
Steroid receptor RNA activator 1 antibody
Steroid receptor RNA activator protein antibody
Anti-SRA1 antibody images
Western blot - SRA1 antibody (ab72407)
All lanes : Anti-SRA1 antibody (ab72407) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 20 µg Lane 2 : Whole cell lysate from Hela cells at 4 µg Lane 3 : Whole cell lysate from MCF-7 cells at 20 µg Lane 4 : Whole cell lysate from 293T cells at 20 µg
Immunoprecipitation/ Western Blot of SRA1.
Lane 1: ab72407 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72407 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 10 seconds.
References for Anti-SRA1 antibody (ab72407)
This product has been referenced in:
McKay DB et al. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. J Mol Biol426:1766-85 (2014).
Read more (PubMed: 24486609) »