Overview

  • Product nameAnti-SRA1 antibody
    See all SRA1 primary antibodies
  • Description
    Rabbit polyclonal to SRA1
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of human SRA1 (NP_001030312.1)

  • Positive control
    • HeLa, MCF7 and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionFunctional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • Tissue specificityHighly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • Sequence similaritiesBelongs to the SRA1 family.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC87674 antibody
    • PP7684 antibody
    • SR A1 antibody
    • SRA antibody
    • SRA1 antibody
    • SRA1_HUMAN antibody
    • SRAP antibody
    • Steroid receptor coactivator antibody
    • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
    • Steroid receptor RNA activator 1 antibody
    • Steroid receptor RNA activator protein antibody
    • STRAA1 antibody
    see all

Anti-SRA1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SRA1 with ab72552 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SRA1 antibody (ab72552) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 20 µg
    Lane 2 : HeLa whole cell lysate at 4 µg
    Lane 3 : MCF7 whole cell lysate at 20 µg
    Lane 4 : 293T whole cell lysate at 20 µg


    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Detection of Human SRA1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72552 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References for Anti-SRA1 antibody (ab72552)

This product has been referenced in:
  • McKay DB  et al. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. J Mol Biol 426:1766-85 (2014). IF . Read more (PubMed: 24486609) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"