Recombinant
RabMAb

Anti-SRA1 antibody [EPR11776] (ab183725)

Overview

  • Product name
    Anti-SRA1 antibody [EPR11776]
    See all SRA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11776] to SRA1
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SRA1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9HD15

  • Positive control
    • Human fetal liver and kidney lysates; Human ovarian carcinoma tissue; 293 and MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-SRA1 antibody [EPR11776] (ab183725)

    Anti-SRA1 antibody (Alexa Fluor® 488) [EPR11776] (ab203155)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/180. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • Tissue specificity
    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • Sequence similarities
    Belongs to the SRA1 family.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC87674 antibody
    • PP7684 antibody
    • SR A1 antibody
    • SRA antibody
    • SRA1 antibody
    • SRA1_HUMAN antibody
    • SRAP antibody
    • Steroid receptor coactivator antibody
    • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
    • Steroid receptor RNA activator 1 antibody
    • Steroid receptor RNA activator protein antibody
    • STRAA1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SRA1 with ab183725 at 1/100 dilution. The slide is counterstained with Hematoxylin.

  • Immunofluorescence analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SRA1 with ab183725 at 1/500 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Anti-SRA1 antibody [EPR11776] (ab183725) at 1/2000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 26 kDa
  • Anti-SRA1 antibody [EPR11776] (ab183725) at 1/10000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000

    Predicted band size : 26 kDa
  • Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling SRA1 with ab183725 at 1/180 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

References

ab183725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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