Overview

  • Product nameAnti-sRANKL antibody
    See all sRANKL primary antibodies
  • Description
    Rabbit polyclonal to sRANKL
  • Tested applicationsSuitable for: WB, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from near the centre of sRANKL

  • Positive control
    • Rat liver tissue lysate Human liver tissue
  • General notes

    This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

Properties

Applications

Our Abpromise guarantee covers the use of ab62516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localizationCytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD254 antibody
    • hRANKL2 antibody
    • ODF antibody
    • OPGL antibody
    • OPTB2 antibody
    • Osteoclast differentiation factor antibody
    • Osteoprotegerin ligand antibody
    • RANKL antibody
    • Receptor activator of NF-kappa-B ligand antibody
    • Receptor activator of nuclear factor kappa B ligand antibody
    • Receptor activator of nuclear factor kappa-B ligand antibody
    • sOdf antibody
    • soluble form antibody
    • TNF-related activation-induced cytokine antibody
    • TNF11_HUMAN antibody
    • Tnfsf11 antibody
    • TRANCE antibody
    • Tumor necrosis factor (ligand) superfamily member 11 antibody
    • Tumor necrosis factor ligand superfamily member 11 antibody
    see all

Anti-sRANKL antibody images

  • Lane 1 : Anti-sRANKL antibody (ab62516) at 0.25 µg/ml
    Lane 2 : Anti-sRANKL antibody (ab62516) at 0.5 µg/ml

    Lane 1 : rat liver tissue lysate
    Lane 2 : rat liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of RANKL expression in paraffin embedded human liver tissue using 5 µg/ml ab62516
  • ICC/IF image of ab62516 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62516, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-sRANKL antibody (ab62516)

This product has been referenced in:
  • Filion TM  et al. Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity expedite the repair of critical femoral defects in rats. Tissue Eng Part A 17:503-11 (2011). IHC-P ; Rat . Read more (PubMed: 20818999) »
  • Walker CG  et al. Osteopontin is required for unloading-induced osteoclast recruitment and modulation of RANKL expression during tooth drift-associated bone remodeling, but not for super-eruption. Bone 47:1020-9 (2010). IHC-P . Read more (PubMed: 20828639) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Bone - Tibia)
Specification Bone - Tibia
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Helder Fonseca

Verified customer

Submitted Sep 25 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step PBS BSA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step None
Sample Human Tissue sections (Skin)
Specification Skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (cell culture supernatants (osteoblast))
Specification cell culture supernatants (osteoblast)
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
Sample Human Tissue lysate - whole (skin)
Specification skin
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Cell (HeLa cells)
Specification HeLa cells
Permeabilization Yes - 0,1% Saponin/PBS
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Jun 28 2013

Thank you for your interest in our products.

Currently we do not carry an anti-RANKL antibody targeting the transmembrane portion of the protein. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam c...

Read More

Thank you for contacting Abcam.

The immunogen sequence for ab62516 is **************.

Please let me know if there is anything else I can help you with.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization Yes - 0,1% Saponin / PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"