• Product name
    Anti-SRB4 antibody
  • Description
    Rabbit polyclonal to SRB4
  • Tested applications
    Suitable for: ChIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.

  • Positive control
    • Saccharomyces cerevisiae extract.



Our Abpromise guarantee covers the use of ab63812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
ELISA Use at an assay dependent dilution.


  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
  • Sequence similarities
    Belongs to the Mediator complex subunit 17 family.
  • Cellular localization
  • Information by UniProt
  • Database links
    • Alternative names
      • MED17 antibody
      • MED17_YEAST antibody
      • Mediator complex subunit 17 antibody
      • Mediator of RNA polymerase II transcription subunit 17 antibody
      • SRB4 antibody
      • Suppressor of RNA polymerase B 4 antibody
      see all

    Anti-SRB4 antibody images

    • All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Saccharomyces cerevisiae extract

      Predicted band size : 78 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • ab63812 used in ChIP.
      Whole cell lysate from yeast.
      X-ChIP cross linking performed for 15 minutes using 1% formaldehyde.
      Real time PCR was used as the detection step.
      Positive control CHA1 gene.
      Negative control SPO20 gene and a nontranscribed region on ChrV.
      ab63812 used undiluted for 16 hours at 4°C.

      See Abreview

    References for Anti-SRB4 antibody (ab63812)

    This product has been referenced in:
    • Young CP  et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28122508) »
    • Paul E  et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . Read more (PubMed: 25368384) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Saccharomyces cerevisiae Cell lysate - whole cell (YEAST)
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde 1%
    Detection step
    Real-time PCR
    Positive control
    CHA1 gene
    Negative control
    SPO20 gene and a nontranscribed region on ChrV

    Dr. Suraiya Ansari

    Verified customer

    Submitted Feb 10 2012

    DISCOUNT CODE: *** Expiration date: [April 15, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab63812 in ChIP and include this code in the “Additional Comments” secti...

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    Thank you for your call today and for your interest in ab63812. Since ab63812 has not been tested in ChIP, I can offer a discount off a future purchase if you buy ab63812 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doe...

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