Overview

  • Product nameAnti-SRB4 antibody - ChIP Grade
  • Description
    Rabbit polyclonal to SRB4 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.

  • Positive control
    • Saccharomyces cerevisiae extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab63812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
  • Sequence similaritiesBelongs to the Mediator complex subunit 17 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • MED17 antibody
      • MED17_YEAST antibody
      • Mediator complex subunit 17 antibody
      • Mediator of RNA polymerase II transcription subunit 17 antibody
      • SRB4 antibody
      • Suppressor of RNA polymerase B 4 antibody
      see all

    Anti-SRB4 antibody - ChIP Grade images

    • All lanes : Anti-SRB4 antibody - ChIP Grade (ab63812) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Saccharomyces cerevisiae extract


      Predicted band size : 78 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • ab63812 used in ChIP.
      Whole cell lysate from yeast.
      X-ChIP cross linking performed for 15 minutes using 1% formaldehyde.
      Real time PCR was used as the detection step.
      Positive control CHA1 gene.
      Negative control SPO20 gene and a nontranscribed region on ChrV.
      ab63812 used undiluted for 16 hours at 4°C.

      See Abreview

    References for Anti-SRB4 antibody - ChIP Grade (ab63812)

    This product has been referenced in:
    • Paul E  et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . Read more (PubMed: 25368384) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ChIP
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (YEAST)
    Specification YEAST
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde 1%
    Detection step Real-time PCR
    Positive control CHA1 gene
    Negative control SPO20 gene and a nontranscribed region on ChrV
    Username

    Dr. Suraiya Ansari

    Verified customer

    Submitted Feb 10 2012

    DISCOUNT CODE: *** Expiration date: [April 15, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab63812 in ChIP and include this code in the “Additional Comments” secti...

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    Thank you for your call today and for your interest in ab63812. Since ab63812 has not been tested in ChIP, I can offer a discount off a future purchase if you buy ab63812 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"