Anti-SRB4 antibody (ab63812)
Key features and details
- Rabbit polyclonal to SRB4
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-SRB4 antibody -
Description
Rabbit polyclonal to SRB4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.
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Positive control
- Saccharomyces cerevisiae extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. -
Sequence similarities
Belongs to the Mediator complex subunit 17 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 856743 Saccharomyces cerevisiae
- SwissProt: P32569 Saccharomyces cerevisiae
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Alternative names
- MED17 antibody
- MED17_YEAST antibody
- Mediator complex subunit 17 antibody
see all
Images
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All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 78 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab63812 has been referenced in 3 publications.
- Young CP et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28122508
- Paul E et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . PubMed: 25368384
- Jeronimo C & Robert F Kin28 regulates the transient association of Mediator with core promoters. Nat Struct Mol Biol 21:449-55 (2014). IP . PubMed: 24704787