Anti-Src (phospho Y418) antibody (ab110777)

Overview

  • Product nameAnti-Src (phospho Y418) antibody
    See all Src primary antibodies
  • Description
    Rabbit polyclonal to Src (phospho Y418)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Src, phosphorylated at Y418, phosphorylated at Y418 and phosphorylated at Y418.

  • Positive control
    • This antibody gave a positive signal in Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab110777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA Use at an assay dependent concentration.

Target

  • FunctionNon-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • Cellular localizationCell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC 2.7.10.2 antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho Y418) antibody images

  • All lanes : Anti-Src (phospho Y418) antibody (ab110777) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Control peptide at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab110777 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

References for Anti-Src (phospho Y418) antibody (ab110777)

ab110777 has not yet been referenced specifically in any publications.

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